What does an endophyte look like? Endophyte profiles of native grasses and shrubs

TitleWhat does an endophyte look like? Endophyte profiles of native grasses and shrubs
Publication TypeConference Proceedings
Year of Publication2006
AuthorsSedillo R.L., Lucero M.E., Barrow J.R., Reyes-Vera I.
Conference NameWildland Shrub Symposium Proceedings
Date PublishedJune 6-8, 2006
Conference LocationCedar City, UT
ARIS Log Number198384
KeywordsDNA, endophytes, fungi, plant tissues
AbstractObligate fungal endophytes often defy detection and isolation by conventional microbiology techniques. Staining methods, including the use of trypan blue and sudan IV have effectively detected endophytes in plant tissues. However, stains fail to distinguish one fungal endophyte from another, thus obscuring the complexity of plant-endophyte communities. We have successfully separated fungal and plant ribosomal DNA sequences by amplifying plant-extracted DNA with polymerase chain reactions, then separating similar sequences with denaturing gradient gel electrophoresis (DGGE). The resulting electrophoregrams produce specific bands unique for each organism present in a specific plant-endophyte community. DGGE also permits detection of bacterial endophytes. Here we compare the diversity of endophytes populations from in vitro cultures of eight Chihuahuan desert grasses and shrubs. These profiles illustrate the complexity of plant systems previously thought of as single organisms.