Volatile composition of <i>Guiterrizia sarothrae </i>(broom snakeweed) as determined by steam distillation and solid phase microextraction

TitleVolatile composition of Guiterrizia sarothrae (broom snakeweed) as determined by steam distillation and solid phase microextraction
Publication TypeJournal Article
Year of Publication2006
AuthorsLucero M.E., Morrison A., Fredrickson E.L., Estell R.E., Richman D.
JournalJournal of Essential Oil Research
Date PublishedApril 1, 2006
ARIS Log Number144600
AbstractGuiterrizia sarothrae (broom snakeweed, snakeweed) is a prevalent species on rangelands throughout much of the western United States. This plant has been intensely studied in order to minimize its negative impact on forage production and livestock health. Although G. sarothrae has been used for centuries to remedy various ailments, scientific investigations of the plant¿s medicinal value are difficult to find in the literature. The objective of this study was to explore the volatile chemical composition of G. sarothrae. Shoots from 88 actively growing plants showing no signs of root borer infestation were selected from five sites on the Chihuahuan Desert of southern New Mexico. Volatiles were extracted from ground, composite tissues by steam distillation and by solid phase microextraction (SPME), then separated and analyzed by gas chromatography with mass spectral and flame ionization detection. Comparing retention indices and mass spectra to known compounds identified ninety chemicals. Retention indices and 70 eV mass spectra are also provided for seven unknowns. Compounds detected varied in quantity between extraction protocols. In oil, cryptone (6.4%) and beta-eudesmol (5.9%) were the only compounds comprising more than 5% of the chromatographic peak area. In samples prepared by solid-phase microextraction, limonene (10.4%), beta-pinene (9.6%), beta-eudesmol (8.0%), sabinene (7.8%), cryptone (6.5%), alpha-pinene (5.5%), and ortho-cymene (5.2%) accounted for 53% of the extracted volatiles. The results presented reveal a complex volatile composition from which unique compounds may still be identified.