An improved protocol for micropropagation of saltbush <i>(atriplex) </i>species

TitleAn improved protocol for micropropagation of saltbush (atriplex) species
Publication TypeJournal Article
Year of Publication2010
AuthorsReyes-Vera I., Lucero M.E., Barrow J.R.
JournalNative Plant Journal
ARIS Log Number247085
Abstract Atriplex griffithsii Standl is a threatened halophytic shrub found in saline soils of isolated regions within the desert basins in Southern New Mexico and Arizona. A related species within the genus, A. canescens, has been successfully micropropagated, suggesting that clonal propagation may offer a strategy to produce large numbers of A. griffithsii plants for conservation, restoration, or research needs. However, previously reported A. canescens micropropagation protocols could not be reproduced with A. griffithsii. Here we evaluated rates of shoot production of A. griffithsii explants exposed to varied growth regulator treatments. Maximal shoot productivity was observed with explants exposed to 1.14 µM indole 3-acetic acid (IAA) + 24.61 µM 6-('-'-dimethylallylamino) purine (2iP). Rooting frequencies of 55% were observed on hormone free medium. Results demonstrate potential to micropropagate clonal A. griffithsii, a species with potential for restoration of saline soils.