Hyperhydricity reversal and clonal propagation of fourwing saltbush (<i>Atriplex canescens, Chenopodiaceae)</i> cultivated <i>in vitro</i>

TitleHyperhydricity reversal and clonal propagation of fourwing saltbush (Atriplex canescens, Chenopodiaceae) cultivated in vitro
Publication TypeJournal Article
Year of Publication2008
AuthorsReyes-Vera I., Potenza C.L., Barrow J.R.
JournalAustralian Journal of Botany
Date PublishedJuly 2008
ARIS Log Number224160
Keywordsbotany, clonal, fourwing, hyperhydricity, saltbush

In vitro propagated shoots of fourwing saltbush [Atriplex canescens (Pursh Nutt)] showed severe symptoms of hyperhydricity. Hyperhydricity is a frequent physiological problem reported in micropropagation of A. canescens and other plant species, and drastically reduces the quality of in vitro cultures. It is exacerbated when plants are cultured in airtight culture vessels on medium formulations with high nitrogen concentrations. We show that the reversion of hyperhydric A. canescens shoots to normal shoots was significantly affected by the presence of inorganic nitrogen in the culture vessel. When the culture vessel was vented or when ammonium nitrate was deleted from Murashige and Skoog basal medium, rates of reversion were significantly higher. While statistically significant differences were evident when comparing vented vs. non-vented treatments for each medium, the modified culture medium with vented closures was consistently the best treatment, showing a total cumulative frequency of 39.7% compared with a total cumulative frequency of 7.1 % observed in the control treatment. Resulting normal shoots also showed significant improvements in further manipulations, including rooting in vitro, transplantation to soil, and survival in native sites.